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50 Years of ERIC
50 Years of ERIC
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Peer reviewed Peer reviewed
ERIC Number: EJ717139
Record Type: Journal
Publication Date: 2004-Mar-1
Pages: 3
Abstractor: Author
Reference Count: 0
ISBN: N/A
ISSN: ISSN-0021-9584
96-Well Plate Colorimetric Assay for K(sub i) Determination of (plusmn)-2-Benzylsuccinic Acid, an Inhibitor of Carboxypeptidase A
Wentland, Mark P.; Raza, Shaan; Yingtong Gao
Journal of Chemical Education, v81 n3 p398 Mar 2004
An appropriate assay to determine the inhibition potency of carboxypeptidase A (CPA) in 96-well format to illustrate how high throughput screening is used in modern drug discovery to identify bioactive molecules is developed. Efforts in developing a colorimetric 96-well plate assay for determination of the K(sub i) for inhibition of CPA by (plusmn)-2-benzylsuccinic acid using Ac-phe-Thiaphe-OH as substrate is reported.
Descriptors: Biochemistry, Science Experiments, Science Instruction, Scientific Methodology, Pharmacology, Molecular Structure
Journal of Chemical Education, Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Web site: http://jchemed.chem.wisc.edu/AboutJCE/contact.html.
Publication Type: Journal Articles; Reports - Research
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers: N/A