In this study, we contrast results from two differential item functioning (DIF) approaches (manifest and latent class) by the number of items and sources of items identified as DIF using data from an international reading assessment. The latter approach yielded three latent classes, presenting evidence of heterogeneity in examinee response patterns. It also yielded more DIF items with larger effect sizes and more consistent item response patterns by substantive aspects (e.g., reading comprehension processes and cognitive complexity of items). Based on our findings, we suggest empirically evaluating the homogeneity assumption in international assessments because international populations cannot be assumed to have homogeneous item response patterns. Otherwise, differences in response patterns within these populations may be under-detected when conducting manifest DIF analyses. Detecting differences in item responses across international examinee populations has implications on the generalizability and meaningfulness of DIF findings as they apply to heterogeneous examinee subgroups.