This paper reports the final development of a scale to measure pre-service teachers' perceptions in three constructs of inclusive education, namely, sentiments or comfort levels when engaging with people with disabilities; acceptance of learners with different needs; and concerns about implementing inclusion. The Sentiments, Attitudes, and Concerns about Inclusive Education Revised (SACIE-R) scale was developed from an initial 60 items and administered through a series of refined surveys. A final 15-item scale was validated using 542 pre-service teachers from nine institutions in four countries including Hong Kong, Canada, India, and the United States. It is posited that the SACIE-R scale will yield valuable information for assisting universities and colleges in preparing more specific training to address the needs of pre-service teachers for working with diverse student populations. (Contains 2 tables.)