Background: College students are vulnerable to risks associated with unhealthy behaviors. Considering the role that colleges play in facilitating lifelong health and wellness behaviors of college students, health-related fitness (HRF) courses are being offered using multiple delivery formats. Purpose: There is a need to better understand the relationship between course delivery format and perceived wellness; thus, the purpose of this study was to assess perceived wellness among college students who self-selected into various delivery formats of a required university HRF course. Methods: The Perceived Wellness Survey was used due to its previously established reliability and validity for college populations. Participants included 378 college students enrolled in a HRF course. Results: Students with higher perceived wellness were more likely to be enrolled in online and hybrid course formats rather than face to face. Discussion: The results of this study provide a better understanding of the perceived wellness of college students enrolled in various course delivery formats of a HRF course that go beyond that of demographic differences. Translation to Health Education Practice: With this information, those delivering HRF courses have the opportunity to capitalize on this trend by tailoring instructional activities accordingly.