The present study was carried out to determine the Science and Technology pre-service teachers' tendencies to explain vitality in a university located in Southeast Anatolia of Turkey in 2010-2011 academic year. The data were collected through the administration of a questionnaire developed by the researcher to 1st and 4th year Science and Technology pre-service teachers (N=123). At the end of the study, it was found that Science and Technology pre-service teachers have a tendency to explain vitality through anthropoformic references such as "vitialist", "animist", "teleologic" types besides biological facts. In the light of these findings, suggestions to teach the biological nature of vitality in a proper manner were made.