Interaction is one of the key variables involved in successful online learning. Previous studies analyzing online interaction are limited in their ability to reveal dynamic aspects of interaction in online learning environments. The aim of this study is to develop and test the multi-level assessment framework for analyzing multi-facets of online interaction. To achieve this purpose, a multi-level assessment framework including micro-assessment and macro-assessment was proposed. The multi-level assessment framework, then was tested with the data collected from an online course. This study suggests that multi-level assessment framework provides us with different kinds of information that helps us to understanding interaction in online environments.