This study examined the gender issue in the performance of students admitted through Universities Matriculation Examination (UME) and pre-degree into the Nigerian Universities. Ex-post facto design of descriptive research was adopted for the study. A proforma was used to collect data from a sample of two hundred and fifty students from the faculties of Science in the Nigerian Universities. Purposive, stratified and proportional sampling techniques were employed in the selection of the sample. Data collected were analyzed using student t-test. Four null hypotheses were generated and tested at 0.05 level of significant. Results of the study revealed that there is no significant difference between the academic performance of male and female students in the Nigerian Universities. Based on the findings, it was recommended that both male and female students should be given equal chances of admission in the UME and Pre-Degree programmes. (Contains 4 tables.)