The enrollment and assessment of performance of National diploma students in Science Laboratory Technology Programme in Nuhu Bamalli Polytechnic, Zaria were studied. The study covered ten academic sessions: 1995-2005. Sample means, percentage and students T-test were employed to analyze the data obtained for the study. The enrollment of male and female students was compared: the results showed lower enrollment and percentage pass for females, the average range of enrollment being 60-70% for males and 15-45% for females. Statistical analysis has revealed significant difference with p values less than 0.05. This shows differences exist in the participation and performance of male and female students of the Science Laboratory Technology Diploma programme in Nuhu Bamalli Polytechnic, Zaria. (Contains 5 tables.)