Locomotive and eating behavior of 52 toddlers was observed at 12 months old in a nursery school and investigated in relation to the acquisition of independent walking. The toddlers who acquired walking ate more by themselves using the hands than the toddlers who did not start walking. This suggested that acquisition of walking was associated with eating behavior. However, onset of walking did not immediately influence the toddlers' interest in other persons or skilful ability to use tools. Sex differences in the toddlers' behavior were minor, but the teachers showed sex bias to the toddlers' behavior.