Sexuality education occurs both formally and informally in the public school system. This study sought to assess sexuality education in Indiana public schools--the classroom curricula as well as students' sexuality-related questions. Data were collected from teachers, nurses, and counselors (N = 399) pertaining to the inclusion of specific topics in sexuality education courses; sexuality-related questions received from students; and reported feelings of comfort and confidence answering the questions. Findings suggest that students' questions address topics not always included in traditional sexuality education curricula. These informal interactions present "teachable moments" where school personnel can provide information that may positively impact students' healthy sexual development. (Contains 7 tables.)