This study evaluates the effectiveness of human anatomy software in face-to-face and online anatomy laboratory classes. Cognitive, affective, and psychomotor perceived learning was measured for students using Pearson Education's Practice Anatomy Laboratory 2.0 software. This study determined that student-perceived learning was significantly greater in the online class environment and was specifically related to psychomotor learning. The findings of this study have implications for future educational practice in the use of advanced digital software for learning in both traditional and online education courses as instructors seek to find better methods to assist their students in developing skills in learning human anatomy.