The purpose of this study is to investigate the question of whether the interaction effects of hypervideo navigation variables (navigation control and navigation links) would affect college students' self-regulated learning just after their learning from instructional hypervideo programs. Navigation control (free navigation or free navigation with advisement) and navigation links (video-based links or video/text-based links) were independent variables in this 2x2 factorial design quantitative study. Instructional content was the same in four experimental treatments: FN/VbL environment (free navigation via video-based links); FN/VTbL environment (free navigation via video/text-based links); FNwA/VbL environment (free navigation with advisement via video-based links); and FNwA/VTbL environment (free navigation with advisement via video/text-based links. Participants in the study were (60) pre-service teachers randomly assigned to one of the four experimental groups. Data analyses were conducted to test the hypotheses derived from research questions. Two-way ANOVA test was used to investigate the main effects of the two independent variables on the ten (10) separate self-regulated learning strategies, and on the overall self-regulated learning strategies (SRL). This study provided statistical evidence that free navigation fosters 100% of the separate and overall self- regulated strategies without the need for advisement at all; that VbL fosters about (91%) of the separate and overall self regulated strategies; and that no effects were found for the interaction between the two independent variables in terms of 7 separate strategies and the overall strategies. (Contains 25 tables and 2 figures.)