The study of caregiver burden is important for those faced with chronic illnesses such as AIDS as caregivers are the first line of care provision, sustaining care recipients in the home. Because perception of burden may vary across caregiver cultural groups, accurate measurement of burden is crucial to offer culturally sensitive services for those providing in-home care. The purpose of this study was to test the factor structure of a rapid assessment instrument, the Latina Caregiver Burden Scale, in a sample of 154 newly immigrated Latina caregivers primarily of Mexican descent. Face-to-face interviews were conducted in Spanish and English by bilingual, bicultural interviewers. The findings suggest that aspects of caregiver burden for these low-income women are tied to factor structures associated with depression and guilt mentality, socioeconomic stress, and stigma linked with HIV/AIDS. Social workers have greater likelihood of accurate cultural assessment of caregiver burden in similar groups when using such research-supported measures.