Abstinence sexuality education (sex ed) is the only federally funded sex ed in the United States. The strict curriculum of this education does not educate American adolescents about safer sex practices and leaves a knowledge gap in these adolescents that follows them into college. The Problem: This project aimed to identify sex knowledge differences between freshmen and senior undergraduate students at a northeast private university. Methods: The Acquisition of Sexual Information Test was taken anonymously online (MonkeySurvey.com) by freshmen and seniors at the selected university. The test covered the four areas of human sexuality: 1) venereal disease; 2) birth control, sexual relations, and reproduction; 3) male biological aspects of sexuality; and 4) female biological aspects of sexuality. Results: The freshmen (n = 52) and senior (n = 40) groups were similar in gender, ethnicity, college attended within the university and type of sex ed received in high school. Overall, the senior students scored significantly higher (p = 0.01) on the test compared to the freshman. More specifically, the seniors scored significantly higher in the sections on "birth control," "sexual relations" and reproduction" (p less than 0.05) and in "male biological aspects of sexuality" (p = 0.05). Conclusions: These data suggest that the seniors may have learned more about sexuality during their time at the university, thus helping to bridge the assumed knowledge gap from high school. (Contains 1 table and 5 figures.)