The purpose of this study was to test and assess the reliability and validity of a modified stress scale in a sample of pharmacy graduate students. The modified stress scale was used as part of a larger, nationwide, study whose aim was to investigate the association of stress, perceived academic success and health-related quality of life among pharmacy administration graduate students in U.S. colleges of pharmacy. A usable response rate of 46.5% (172/370) was obtained. A principal components analysis with varimax orthogonal rotation resulted in a 29-item, eight component solution (accounting for 66.2% of the explained variance). The eight components were named: (1) fear of failing stress, (2)academic stress, (3) world stress, (4) time stress, (5) stress about feedback, (6) student-faculty interaction stress, 7) economic stress and 8) environmental stress. The Cronbach coefficient alpha value of .90 for the 29-item scale indicated high reliability and internal consistency. The predictive validity results were significant and in the expected direction providing evidence of the validity of the modified stress scale. The modified stress scale is a reliable and valid measure of assessing stress in pharmacy administration graduate students and may be useful in identifying areas of stress that may be present in graduate students from other disciplines. (Contains 4 tables.)