This research explored the relationship of educational entrepreneurism and organizational culture in the creation and evolution of academic centers within one Midwestern land-grant university facing resource constraints. Particular attention was given to: (a) synthesizing current entrepreneurial and organizational culture and evolution theory as it relates to postsecondary education; (b) ascertaining the implications of a qualitative, comparative, case study approach to analyzing entrepreneurism and organizational culture and evolution within a university setting and (c) presenting the application of and reflection on a qualitative, comparative, case study approach to researching educational entrepreneurism and organizational culture and evolution as they relate to the creation of two, not-for-profit centers. The findings of this study indicate that these centers have utilized entrepreneurial processes within an academic and non-academic setting to serve a public interest. Primary factors supporting entrepreneurial processes in these academic centers include: (a) mission focus; (b) remaining objective to serve multiple constituents; (c) cultivating resources, both people and funding; (d) building leaders from within; (e) commitment and (f) champions. The relationship of organizational culture with these factors was found to be the key for continuous innovation. [The dissertation citations contained here are published with the permission of ProQuest LLC. Further reproduction is prohibited without permission. Copies of dissertations may be obtained by Telephone (800) 1-800-521-0600. Web page: http://www.proquest.com/en-US/products/dissertations/individuals.shtml.]