In recent years, postsecondary instructors have recognized the potential of wikis to transform the way students learn in a collaborative environment. However, few instructors have embraced in-depth student use of chemistry software for the creation of interactive chemistry content on the Web. Using currently available software, students are able to design, build, and describe computational molecular models with a high degree of independence. For a second-semester organic chemistry course with biochemistry applications, we designed and implemented a wiki-based project that involved student development of Web pages presenting the mechanism of action of a molecule of their choice. Student feedback supports the value of the project as a means to help students apply course content to biochemical applications. Organic chemistry instructors may benefit from the experience we gained while designing and implementing an organic chemistry wiki project. (Contains 3 tables and 2 figures.)