Students (N = 1,146) from five Historically Black Colleges and Universities in Texas, Louisiana, and Oklahoma participated in this study. Although students report a moderate level of HIV knowledge, they are deficient on three items related to the role of Nonoxynol-9 on HIV transmission, role of prior STD history on HIV transmission, and meaning of a positive blood HIV test. While women scored significantly higher on HIV knowledge scores (Men = 6.19, Women = 6.47, t(519) = -2.887, p = 0.004), more men reported using HIV testing services on campus (Men = 0.33, Women = 0.23, t(950.545) = 3.701, p = 0.0005), and being more aware of HIV testing services in their community (Men = 0.72, Women = 0.65, t(1056.271) = 2.343, p = 0.019). Significant differences were found between men and women on all four HIV testing factors of confidentiality, nonjudgmental staff, convenience and cost (Men = 13.24, Women = 14.10, t(372.843) = -3.428, p =0.001). Women perceive a stronger support for condom use on campus than do men (Men = 23.61, Women = 25.88, t(520) = -4.190, p = 0.0005). Overall students do not perceive themselves at great risk for HIV; and there were no overall gender differences on perception of risk. (Contains 2 tables.)