The information world in which library and information science (LIS) students study and are being educated is in a state of constant change. As Web 2.0 is becoming an important and central topic in our information world, the research reported here explores whether LIS students are familiar with technological changes and innovations, and whether they make use of the different Web 2.0 applications. The research examined whether personality characteristics (learning strategies, resistance to change and cognitive appraisal), as well as learning facilitators such as motivation, capacity and importance of studying and integrating different applications of Web 2.0 in the future, influenced LIS students' perceptions towards Web 2.0 use. Different questionnaires were distributed to 160 randomly selected Israeli students from three different LIS programmes. The research revealed a moderate tendency of LIS students to use Web 2.0 applications. It was found that personality characteristics as well as learning facilitators influenced LIS students' perceptions towards Web 2.0 use. (Contains 3 tables and 1 figure.)