The current study presents a newly developed measurement: the TEMO (Teacher and Motivation) scale, which assesses adolescent students' perception of liked and disliked teachers and the resulting impact on their academic motivation. A total of 1,088 students from secondary schools in Germany participated in this study. To explore the underlying factor structure of the 16 TEMO items, a two-stage approach was used with structural equation modeling: (a) exploratory factor analyses (EFA) and (b) confirmatory factor analyses (CFA). Factor analyses supported a four-factor solution with acceptable internal consistency for the 16 items: (a) student's motivation based on liking the teacher (SML), (b) student's motivation based on teacher's commitment (SMC), (c) student's motivation based on disliking the teacher (SMD), and (d) student's motivation based on teacher's lack of commitment (SMLC). Students' scores on the TEMO were significantly associated with different aspects of academic motivation. Results indicate that the TEMO scale is a robust instrument that directly assesses the association between students' liking/disliking of a teacher and their motivation in class, based on understanding emotions as a driving force of motivation. Teacher and school psychologists can use this instrument to foster student's motivation and improve teacher-student relationships through uncovering underlying emotions.