ERIC Number: EJ918442
Record Type: Journal
Publication Date: 2010-Jul
Pages: 3
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Confirming the 3D Solution Structure of a Short Double-Stranded DNA Sequence Using NMR Spectroscopy
Ruhayel, Rasha A.; Berners-Price, Susan J.
Journal of Chemical Education, v87 n7 p732-734 Jul 2010
2D [superscript 1]H NOESY NMR spectroscopy is routinely used to give information on the closeness of hydrogen atoms through space. This work is based on a 2D [superscript 1]H NOESY NMR spectrum of a 12 base-pair DNA duplex. This 6-h laboratory workshop aims to provide advanced-level chemistry students with a basic, yet solid, understanding of how the 3D structure of a double-stranded DNA sequence can be determined using 2D [superscript 1]H NOESY NMR spectroscopy and, hence, how structural perturbations induced by DNA binding agents such as platinum anticancer drugs are studied. Students are supplied with various sections of the 2D [superscript 1]H NOESY spectrum of the DNA sequence. Using these, as well as a molecular model as a visual aid and tool for measuring interproton distances, they are able to sequentially assign the structure of the DNA. (Contains 2 notes and 2 figures.)
Descriptors: Spectroscopy, College Science, Chemistry, Advanced Courses, Genetics, Cancer, Drug Therapy, Science Experiments, Molecular Structure, Models
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A