The study assessed users' information needs and satisfaction in selected seminary libraries in Oyo State, Nigeria. This paper employed the descriptive survey research design, whereby the expost-facto was employed with a sample size of three hundred (300) participants, selected from six seminaries located in Ibadan, Oyo and Ogbomoso, all in Oyo state of Nigeria. The study used purposive sampling technique. A structured questionnaire was used to elicit information from the respondents and data obtained were analyzed using descriptive statistics of frequency counts, percentage and charts. The results indicated that the information materials needed by users of selected seminary libraries in Oyo State are availability of journals, non-book materials such as electronic databases, interlibrary loan services as well as internet access. These are largely scarce in the selected seminary libraries. They were either not available or not sufficient or commensurate with the number of users available in such libraries; the users do not always achieve their aims of visiting the libraries; major constraints encountered by users were inadequate hours of opening of libraries; lack of adequate and current issues of journals; problems related to accessibility issues as well as lack of internet connectivity within the library premises; and that the seminary libraries are not well equipped electronically to meet the needs and satisfaction of their users most especially in the twenty first century librarianship. Recommendations were made based on the findings of this study.