ERIC Number: EJ981260
Record Type: Journal
Publication Date: 2012-Feb
Pages: 3
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Microplate Bioassay for Determining Substrate Selectivity of "Candida rugosa" Lipase
Wang, Shi-zhen; Fang, Bai-shan
Journal of Chemical Education, v89 n3 p409-411 Feb 2012
Substrate selectivity of "Candida rugosa" lipase was tested using "p"-nitrophenyl esters of increasing chain length (C[subscript 1], C[subscript 7], C[subscript 15]) using the high-throughput screening method. A fast and easy 96-well microplate bioassay was developed to help students learn and practice biotechnological specificity screen. The kinetic constants V[subscript max] and K[subscript m] of each substrate were compared to indicate the lipase activity and selectivity to different substrates. In this experiment, students were exposed to the core concepts of Michaelis-Menten kinetic analysis and learned the principles and techniques of studying substrate selectivity, including the use of a microplate reader and the associated software, the layout of bioassays, and the multichannel-repeater pipetting. (Contains 1 table, 1 figure and 1 scheme.)
Descriptors: Kinetics, Science Instruction, College Science, Undergraduate Study, Chemistry, Spectroscopy, Science Experiments, Scientific Principles
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A