For the past six years, the American College Health Association (ACHA) has had two cooperative agreements from the US Centers for Disease Control and Prevention's (CDC) Division of Adolescent and School Health. Both cooperative agreements focus on the prevention of HIV (human immunodeficiency virus) among college students, particularly for students of color and gay, lesbian, bisexual, transgender, and questioning (GLBTQ) students. In this article, the authors discuss the connection between HIV and culture, define cultural competency, discuss the importance of recognizing heterogeneity within cultural groups and the multiple identities of individuals, and offer recommendations for building a culturally competent college health program. (Contains 6 online resources.)