The purpose of the study was to determine the relationship of physical activity (PA) level and healthy life-style behaviors in academic staff in Bartin University, Turkey. The short form of International Physical Activity Questionnaire was administered for the determination of physical activity level of academic staff. Their PA levels were categorised as inactive, minimally active, and physically active by using MET method. For the determination of healthy life-style behaviors, Health-promoting Life-style Profile Questionnaire was used with self-actualization (SA), health responsibility (HR), exercise (E), nutrition (N), interpersonal support (IS), and stress management (SM) subscales. Results of Pearson's Product Moment Correlation analyses indicated significant positive correlations between total physical activity level and exercise (r = 0.52; p<0.01) and healthy life-style behaviors (r = 0.47; p<0.01) among the study subjects. There was no significant correlation between healthy life-style behaviors and sport index of physical activity questionnaire (p>0.05). The findings of the study indicated that healthy life-style behaviors were not indicators of physical activity level of academic staff.