Prospective elementary teachers hold preconceived ideas about elementary school students' attitudes toward mathematics. We found that there exists a gender bias with prospective teachers expecting girls to have negative attitudes toward mathematics and boys more likely to have positive attitudes toward mathematics. We found that these expectations exist for both prospective teachers in a traditional undergraduate degree program and prospective teachers in an alternative licensure graduate degree program. We also found that these expectations do change with the completion of a mathematics methods course and classroom experiences.