This article describes a study that investigated preservice teachers' understandings and self-efficacy related to lesbian, gay, bisexual, transgender, or queer (LGBTQ) students and families. The preservice teachers indicated a broad range of understandings in relation to LGBTQ terms. They reported a relatively high sense of self-efficacy in working with LGBTQ students, with slightly lower self-efficacy in identifying biases in curricular materials and school contexts and in teaching LGBTQ content in their classrooms.