A needs assessment survey was developed to assess the knowledge and attitudes of poultry vendors at farmers' markets in Pennsylvania, on food safety, regulation, and poultry production. Vendors were administered a 32-question paper survey, in person, during market hours. The results revealed critical vendor practices and identified important vendor knowledge gaps and attitudes on food safety and poultry processing. The data obtained from the study will aid in the development of future farmers' market research, as well as generating training and outreach materials on food safety for vendors selling meat and poultry products at farmers' markets.