To test the effects of octave and timbre on tuning accuracy, four stimuli--B-flat 4 sounded by flute, oboe, and clarinet and B-flat 2 sounded by tuba--functioned as reference pitches for high school wind players (N = 72). The two stimulus octaves combined with participants' assigned tuning notes created soprano, tenor, and bass tuning groups. All participants tuned to each instrument. Results indicated no effect due to tuning group. There was a significant difference due to stimulus. Participants' responses were more out of tune to the tuba stimulus than to the oboe, clarinet, and flute stimuli, which were not different from each other. There was no difference in the distribution of in-tune, sharp, and flat responses across tuning stimuli, a result that differs from the "preference for sharpness" effect in previous research. Verbal and performance responses to the tuba, oboe, and flute stimuli revealed misconceptions between participants' perceptions of tuning difficulty and actual performance difficulty and favored the use of oboe and flute as tuning references. Most of the participants (82%) reported tuning to the tuba as the prevalent approach to mass tuning in their school bands. (Contains 2 tables and 1 note.)