The purpose of this study was to examine elementary physical education teachers' content knowledge of physical activity and health-related fitness. Sixty-four female and 24 male teachers completed the Appropriate Physical Activity and Health-Related Fitness test. Descriptive statistics results indicated that the mean percentage score for the test was 57.6%. Results from the ANOVA indicated gender and level of education were unrelated to content knowledge of physical activity and health-related fitness. Years of teaching experience was found to significantly influence content knowledge of physical activity and health-related fitness. It is recommended that ongoing professional development activities be provided to improve the teaching of physical activity and health-related fitness in schools. (Contains 4 tables.)