An Undergraduate Laboratory Experiment that Utilizes a Glass Fiber Filter Assay to Determine the Steroid Specificity and Equilibrium Binding Properties of Glucocorticoid Receptors.

John, Nancy J.; Firestone, Gary L.

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Peer reviewed

ERIC Number: EJ364125

Record Type: Journal

Publication Date: 1987

Pages: N/A

Abstractor: N/A

ISBN: N/A

ISSN: N/A

EISSN: N/A

An Undergraduate Laboratory Experiment that Utilizes a Glass Fiber Filter Assay to Determine the Steroid Specificity and Equilibrium Binding Properties of Glucocorticoid Receptors.

John, Nancy J.; Firestone, Gary L.

Biochemical Education, v15 n4 p209-11 Oct 1987

Describes two complementary laboratory exercises that use the glass fiber assay to assess receptor specificity and hormone binding affinity in rat liver cytoplasmic extracts. Details the methods, materials and protocol of the experiments. Discusses the basic concepts illustrated and the feasibility of using the experiments at the undergraduate level. (CW)

Descriptors: Biochemistry, College Science, Higher Education, Laboratory Procedures, Science Curriculum, Science Education, Science Experiments, Science Instruction, Teaching Methods, Undergraduate Study

Publication Type: Journal Articles; Reports - Descriptive; Guides - Classroom - Teacher

Education Level: N/A

Audience: Practitioners; Students

Language: English

Sponsor: N/A

Authoring Institution: N/A

Grant or Contract Numbers: N/A

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