This study examines pre-service teachers' conception about teaching and learning using the perspectives of Traditional Teaching (TT) and Constructivist Teaching (CT). Using the Teaching and Learning Conceptions Questionnaire (TLCQ) by Chan and Elliot (2004), data were collected from 460 pre-service teachers in Turkey through an online questionnaire. An exploratory factor analysis and confirmatory factor analysis were conducted to validate the TLCQ that was translated into Turkish. The results revealed a factor model, with nine items. Correlations between CT and TT was moderate suggesting a distinct relationship. (Contains 1 figure.)