This study examines the hydrolysis of lignocellulose extracted from poultry manure for the purpose of investigating low-cost feedstocks for ethanol production while providing an alternative solid waste management strategy for agricultural livestock manures. Poultry manure underwent various pretreatments to enhance subsequent enzymatic hydrolysis including untreated, alkaline pretreatment with 0.5N KOH, drying, and grinding. The KOH-treated, dried, and grinded poultry manure yielded the highest glucose conversions. When poultry manure without pretreatment was hydrolyzed at 40 [degrees Celsius] with an enzyme loading 400 units/g feedstock, 7.1 [plus or minus] 0.3% was converted to glucose in 24 hours. This increased to 27.6 [plus or minus] 1.2% when a KOH pretreatment followed by drying and grinding was applied. Fourier transform infrared spectroscopy analyses were used to examine differences in the composition of the pretreated feedstock before and after enzymatic hydrolysis. These indicate that the cellulose content in pretreated poultry manure was removed during enzymatic hydrolysis. (Contains 4 figures and 2 tables.)