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ERIC Number: EJ979769
Record Type: Journal
Publication Date: 2012-Jan
Pages: 5
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Microfluidic Gel Electrophoresis in the Undergraduate Laboratory Applied to Food Analysis
Chao, Tzu-Chiao; Bhattacharya, Sanchari; Ros, Alexandra
Journal of Chemical Education, v89 n1 p125-129 Jan 2012
A microfluidics-based laboratory experiment for the analysis of DNA fragments in an analytical undergraduate course is presented. The experiment is set within the context of food species identification via amplified DNA fragments. The students are provided with berry samples from which they extract DNA and perform polymerase chain reaction (PCR) with strawberry-specific primers. The resulting PCR products are analyzed using the Agilent Bioanalyzer. Using the raw data, the students are tasked to identify the strawberry sample. This course serves as a practical introduction into microfluidic-based capillary gel electrophoresis as well as a primer for biomolecular DNA analysis. (Contains 1 table and 4 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A