When the Mayo Effect video went live on the Mayo intranet in June 2010, it was very well received at Mayo Clinic. The message in the video was so effectively delivered that it became an instant sensation across the institution. The video contains about 461 words. In such a short video, every part of its architectural design, whether it is visual, verbal, or audio, is effectively deployed. This paper will examine the Mayo Effect video in terms of its design, discourse, and effectiveness of communication using the principle of Multimedia, Modality, Personalization and Voice, and Segmenting defined by Clark & Mayer and Sweller. The purpose of this paper is to show that a successful e-Learning piece, whether it is a video presentation or an e-Learning course, comes from the effective design and strategic use of visual, verbal, and audio representations.