This article describes a forensic DNA science laboratory exercise for advanced high school and introductory college level biology courses. Students use a commercial genotyping kit and genetic analyzer or gene sequencer to analyze DNA recovered from a fictitious crime scene. DNA profiling and STR genotyping are outlined. DNA extraction, PCR, and capillary electrophoresis are reviewed. Acquisition of specific reagents and equipment and recommendations for establishing partnerships with companies or laboratories are discussed. Ideas for alternative activities, safety considerations, and personal privacy issues are included.