Taiwan is currently attempting to develop itself into a twenty-first century tourist hub to take advantage of today's thriving global tourism economy. In the coming years, Taiwan anticipates an urgent demand for tour guides, and there is a clear need for training solutions that can serve a rapidly growing population. Computer-mediated virtual 3D situated tourist sites (3D-STS) may provide an efficient solution. This study investigates users' perspectives on a specially developed tour-guide training course employing 3D-STS technology. After participants completed the course, their responses to a survey questionnaire and follow-up interviews were compiled for statistical analysis. The results indicated that the overall experience of 3D-STS was satisfactory for trainees and showed a significant difference in the perspectives on 3D-STS of male and female trainees. However, one essential limitation of the study, that is, insufficient familiarity with the multiple advanced technologies, must be acknowledged. This could be a major obstacle for some users, interfering with their ability to access and process e-learning content. For more effective training outcomes for tour guides, the dynamic nature of 3D-STS as well as the characteristics of course content for tour guides should be considered carefully.