Purpose: To examine the effect of organizational culture and climate on depressive symptoms among nursing home residents. Design and Methods: Using a pooled cross-sectional design, this study examines a sample of 23 nursing homes, 1,114 employees, and 5,497 residents. Depressive symptoms were measured using the Minimum Data Set, Depression Rating Scale. Organizational culture and climate were measured using the Organizational Social Context Scale. Data were analyzed using hierarchical linear modeling. Results: Depressive symptoms were associated with 2 dimensions of organizational culture (proficiency and resistance), 3 dimensions of climate (stress, engagement, and functionality), geographic location, facility size, staffing ratios, and impairments in activities of daily living and cognition. Implications: Findings suggest that values and beliefs that guide behavior in an organization and employees' perceived impact of the work environment of their well-being have a significant impact on resident mental health.