Objective: This study investigated body weight and nutritional behavior perceptions among Division II collegiate athletes. Participants: The sample was composed of 155 collegiate athletes who responded to a survey. Methods: Data were self-reported by athletes via questionnaire. Independent-sample t tests were used to identify significant gender differences related to body weight perceptions, nutritional practices, and ideal weight difference (IWD). An alpha level of 0.05 was used to determine statistical significance. Results: Mean ± SD female and male IWD was -6.8 ± 8.8 and 1.3 ± 12.3 lbs, respectively (p < 0.001). Significant differences in level of agreement regarding perceived body weight effects on performance and dietary practices existed between genders. Conclusions: Significant gender differences may exist regarding body weight perceptions and nutritional practices among collegiate athletes. Deliverance of gender-specific educational information about proper nutritional practices is warranted. To properly tailor interventions to specific sports, research with larger sample sizes is needed.