In the current study of 353 science and engineering faculty members, we examined whether three types of gender-based mistreatment might "chill" individuals' perceptions of the professional climate, which might in turn undermine satisfaction with their jobs. We also tested gender differences in these relationships. Results indicated that for women, the relationship between gender discrimination (e.g., unequal access to resources) and job satisfaction was mediated by scholarly alienation and a negative workplace climate; gender derogation (e.g., disparaging comments) was related to organizational sexism toward women (OSTW), which was associated with perceptions of scholarly alienation and a negative workplace climate; these perceptions in turn predicted lower job satisfaction. For men, gender derogation was indirectly related to job satisfaction via scholarly alienation, and OSTW was indirectly related to job satisfaction via both climate variables. Analyses indicated that most of these indirect effects were stronger for women than men. We discuss these results for both sexes and suggest reasons why men's climate perceptions may be "chilled" by exposure to sexism toward women. We also discuss implications for individuals working with women in male-dominated environments, such as organizational administrators and clinical practitioners.