This quantitative study investigated and compared the development of professional learning communities in schools located in two Chinese cities, namely, Shanghai and Mianyang. The two cities have significant differences in terms of educational, economic, social, and cultural development. While Shanghai is a directly controlled municipality in East China, Mianyang is a city of Sichuan Province in Southwest China. Based on the literature review and an analysis of the Chinese context, the authors developed a questionnaire to explore and compare the professional learning community (PLC) practices of schools in the two cities. Findings show that schools in Mianyang had more PLCs practices than those sampled schools in Shanghai, in terms of collaborative learning and facilitative leadership. Such differences could be explained by the educational, economic, social, and cultural differences between the two cities. Practical implications for school development in both Shanghai and Mianyang as well as suggestions for future research are provided.