The Children's Hand-Skill ability Questionnaire (CHSQ) is a new parent-report questionnaire that assesses children's manual ability in three domains: leisure and play, school/education, and activities of daily living. The CHSQ can be used with children presenting with a range of disabilities and works as a companion assessment before detailed performance-based observations of hand skill are completed. This study further investigated the internal and external construct validity of the CHSQ. Participants included 53 Australian children and 70 Taiwanese children with known disabilities ranging age from 2 to 12 years. Rasch analysis results confirmed the appropriateness of the CHSQ's 3-level rating scale in this combined group of children. Twenty-one of the 22 items in the CHSQ formed three individual unidimensional scales representing with the domains of leisure and play, school/education, and activities of daily living. The CHSQ also exhibited sufficient person-response validity (all the three domains) and item-difficulty range (all but one domain) when applied to children with disabilities. Less than half of the CHSQ items were found to exhibit differential item functioning with regards to gender (4 items) and cultural difference (7 items). In addition, moderate to high correlations (0.61 less than or equal to Pearson's r coefficients less than or equal to 0.76, p less than 0.01) were found with the assessments of self-care and hand skill performance. Therefore, the CHSQ exhibits preliminary evidence of construct validity for its clinical use in obtaining children's manual ability based on parent-report information. (Contains 1 figure and 4 tables.)