Developing scientifically literate students who understand the socially contextualized nature of science and technology is a national focus of science education reform. Science educators' perceptions of risks and benefits of new technologies (such as biotechnology) may shape their instructional approaches. This study examined the perceived risk of biotechnology of four groups of science educators: pre-service science teachers, in-service science teachers, biology graduate teaching assistants, and biology professors (n = 91). Data sources included a survey instrument and card sort task designed to determine the respondents' structure of risk perception and factors contributing to this structure. The perceptions of the four educator groups were compared and contrasted along these dimensions. Results showed that the teacher groups were similar along many aspects of risk perception, but university professors were more likely to view the more subtle "gray areas" between biotechnology risks. The results are discussed in the context of understanding teacher risk perception on science pedagogical practice as well as the role of content knowledge and teaching experience on risk perception formation.