The purpose of this ITEMS module is to provide an introduction to differential item functioning (DIF) analysis using mixture item response models. The mixture item response models for DIF analysis involve comparing item profiles across latent groups, instead of manifest groups. First, an overview of DIF analysis based on latent groups, called latent DIF analysis, is provided and its applications in the literature are surveyed. Then, the methodological issues pertaining to latent DIF analysis are described, including mixture item response models, parameter estimation, and latent DIF detection methods. Finally, recommended steps for latent DIF analysis are illustrated using empirical data.