This study examined the psychometric properties of the Teacher Well-Being Scale, which assesses three factors of teachers' work-related well-being: workload, organizational, and student interaction well-being. With a sample of Canadian teachers, results confirmed the reliability, approximate normality, and factor structure of the scale; provided support for a higher order factor of teacher well-being; showed the instrument functioned similarly across different sociodemographic subgroups; and demonstrated the well-being factors were related as expected with external constructs of teacher stress, job satisfaction, and general well-being. Combined, these analyses provide support for the use of the instrument as an assessment of teacher well-being and evidence of the importance of teacher well-being for other teacher outcomes. Implications for research and practice are discussed.