Three studies were conducted to develop and validate a theoretically derived multidimensional inventory of females' sexual self-conceptions ("sexual subjectivity"). Study 1 revealed five factors on the Female Sexual Subjectivity Inventory (FSSI): sexual body-esteem, three factors of conceptions and expectations of sexual desire and pleasure (self, partner, and self-efficacy), and sexual self-reflection. A shorter revised version of the FSSI was tested in Study 2. In Study 3, a confirmatory factor analysis indicated a good fit to the data. The FSSI had a sound factorial structure and high reliability. Significant associations between the five scales in the FSSI and sexual self-awareness, safe sex self-efficacy, and sexual anxiety provided evidence of validity. Some FSSI scales were also associated with self-silencing in close relationships, resistance to sexual double standards, and self-esteem. Sexual subjectivity is a complex intraindividual phenomenon that includes cognitive and emotional components, some of which can be assessed with the FSSI scales.