ERIC Number: EJ1013562
Record Type: Journal
Publication Date: 2013-Apr
Pages: 6
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Flow Injection Analysis and Liquid Chromatography for Multifunctional Chemical Analysis (MCA) Systems
Mayo, Ana V.; Loegel, Thomas N.; Bretz, Stacey Lowery; Danielson, Neil D.
Journal of Chemical Education, v90 n4 p500-505 Apr 2013
The large class sizes of first-year chemistry labs makes it challenging to provide students with hands-on access to instrumentation because the number of students typically far exceeds the number of research-grade instruments available to collect data. Multifunctional chemical analysis (MCA) systems provide a viable alternative for large-scale instruction while supporting a hands-on approach to more advanced instrumentation. This study describes how the capabilities of MCA systems are extended to introduce liquid chromatography (LC) and flow injection analysis (FIA) in undergraduate laboratories. A semi-micro plastic cuvette with a Teflon tubing insert is fashioned as the flow cell for a MCA absorbance-fluorescence detector. Two MCA systems, Vernier and MeasureNet, are used in two unique experiments demonstrating the detection of salicylate in aspirin tablets by FIA and the LC separation of a mixture of riboflavin and fluorescein. Both instruments, composed of a syringe pump, T-injection valve, and the MCA detector, operated in the kinetic mode, are rugged and inexpensive permitting student construction, if desired. (Contains 2 tables, 6 figures, and 1 scheme.)
Descriptors: Science Instruction, College Science, Chemistry, Science Laboratories, Spectroscopy, Science Experiments, Teaching Methods, Laboratory Equipment
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A