ERIC Number: EJ1003087
Record Type: Journal
Publication Date: 2012-Nov
Pages: 4
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
A Discovery-Oriented Approach to Solid-Phase Peptide Synthesis
Bockman, Matthew R.; Miedema, Christopher J.; Brennan, Brian B.
Journal of Chemical Education, v89 n11 p1470-1473 Nov 2012
In this discovery-oriented laboratory experiment, students use solid-phase synthesis techniques to construct a dipeptide containing an unknown amino acid. Following synthesis and cleavage from the polymeric support, electrospray ionization-mass spectrometry is employed to identify the unknown amino acid that was used in the peptide coupling. This experiment, performed in two, 3-h laboratory periods, provides an opportunity for students to learn the technique of solid-phase peptide synthesis and to critically evaluate data that they generate in the laboratory. The experiment is suitable for second-semester organic chemistry students as they learn methods for the formation of amide bonds or upper-level biochemistry students as they learn about the primary structures of proteins. (Contains 1 figure and 4 schemes.)
Descriptors: Biochemistry, Organic Chemistry, Laboratory Experiments, College Science, Undergraduate Study, Science Laboratories, Spectroscopy, Scientific Concepts, Molecular Structure
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A