In Saudi Arabia gender segregation is a cultural practice that occurs across all public and private domains. This segregation has shaped the lives of Saudi citizens and is driven socially through cultural and religious discourses and politically through regulation and policy. For Saudi students undertaking their education in western countries, the transitioning experience can be challenging. This paper draws on research conducted in Australia that has attempted to explore and understand this transitioning experience. The aim of the study was to highlight the dialectical relationship between participants' cultural identity and the acculturation experience of cross-cultural contact. The findings indicate that Saudi students' cultural identity has a significant influence on the experience of being in a mixed-gender environment. Findings also suggest a relationship between current experience and cultural identity. Drawing on in-depth interviews, the paper provides context to help understand the transitioning experience of Saudi students. (Contains 1 figure.)