There are many educational interventions being implemented to address workforce issues in the field of nanotechnology. However, there is no instrument to assess the impact of these interventions on student awareness of, exposure to, and motivation for nanotechnology. To address this need, the "Nanotechnology Awareness Instrument" was conceptualized. This paper is a progress report of the instrument development process. Version 1 of the instrument was administered to 335 first-year students majoring in food and agriculture fields in a pre-post fashion relative to a brief exposure to nanotechnology in the classroom. Following item analysis of Version 1 responses, a revision of the instrument was completed. Version 2 was administered to 1,426 first-year engineering students for the purpose of conducting item and factor analyses. Results indicate that the "Nanotechnology Awareness Instrument" shows potential to provide valid information about student awareness of, exposure to, and motivation for nanotechnology. The instrument is not a valid measure of nano-knowledge and this subscale was dropped from the final version of the instrument. Implications include the use of the instrument to evaluate programs, interventions, or courses that attempt to increase student awareness of nanotechnology. Further study is necessary to determine how the "Nanotechnology Awareness Instrument" functions as a pre-post measure.